FIGURE 3.

α-SMA expression in UUO-induced kidney injury. A, immunoblotting for α-SMA expression in kidneys from obstructed wild-type and PAR2^−/−, at day 7 post-UUO. Sham operated and contralateral kidneys are used as controls. B, histogram showing densitometry analysis of α-SMA expression normalized to GAPDH in wild type or PAR2^−/− kidneys at days 7 and 14 post-UUO (mean ± S.E.; *, p < 0.05, n = 4–8). C, representative image showing kidney α-SMA expression detected by immunofluorescence for each group at day 7 post-UUO.