Figure 3.

The modified TBNK assay allows rapid quantitation of APC subsets, consistent with conventional methods. (A) Correlation between monocyte and DC counting performed using the 6-color immunophenotyping panel modified by addition of CD14 vs. the standard 8-color DC panel (n = 24). Red dots indicate results for case 1 (DCML deficiency). Green dots indicate case 2 (BPDCN). Linear regressions, r² and p values stated on the plots exclude these outlier cases. (B) Correlation between mDC and pDC counting using the 6-color panel modified with CD14 and CD304 vs. the standard 8-color DC panel (n = 14). (C) The modified TBNK assay applied to case 1 (DCML deficiency). Note the scarcity of CD3⁻ cells relative to CD3⁺ T cells. This arises from reduction in B cells, absence of NK cells, and virtual absence of all monocyte and DC subsets. The small population of CD3⁻ CD4^bright cells gated out in the initial plot are activated T cells with reduced CD3 expression. (D) The modified TBNK assay applied to case 2 (BPDCN).