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. 2013 Dec 27;4:495. doi: 10.3389/fimmu.2013.00495

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Copyright © 2013 Jardine, Barge, Ames-Draycott, Pagan, Cookson, Spickett, Haniffa, Collin and Bigley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD4 is expressed on both DR low and DR bright populations following G-CSF mobilization. The modified TBNK assay allows clear discrimination of lymphocyte, monocyte, and DC populations in left-shifted blood. (A) The lineage DR plot used for 8-color DC profiling shows DR^bright and DR^low populations of monocytes and DCs in G-CSF mobilized blood. (B) CD4 expression is highest on lin⁻ DR^bright cells but also positive on lin⁻ DR^low cells. (C) The modified TBNK assay applied to G-CSF mobilized peripheral blood stem cells (PBSC) permits effective discrimination of lymphocyte, monocyte, and DC populations. (D) Correlation between 8-color and modified TBNK methods for DCs and monocytes in PBSC.