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. 2004 Mar 24;101(14):5012–5017. doi: 10.1073/pnas.0307137101

Fig. 2.

Fig. 2.

BacA affects the lipid-A fatty-acid modifications of both S. meliloti and B. abortus. (A) MALDI-TOF MS of S. meliloti wild-type Rm1021 lipid A; major species [M - H]- 2039 and [M - H]- 1939. (B) Same as A, except S. meliloti Rm1021 bacA null mutant; major species [M - H]- 1516 with lesser amounts of [M - H]- 2039 and [M - H]- 1939. (C) Fatty-acid compositions of S. meliloti wild-type Rm1021 (filled bars) and its bacA null mutant (open bars) lipid A as determined by GC-MS. (D) MALDI-TOF MS of B. abortus wild-type 2308 lipid A; major species [M - H]- 2201 and [M - H]- 2116. (E) Same as D, except B. abortus 2308 bacA null mutant; major species [M - H]- 1495, [M - H]- 1467, [M - H]- 2202 and [M - H]- 2116. (F) The fatty-acid composition of B. abortus wild type (filled bars) and bacA null mutant (open bars) lipid A, as determined by GC-MS. For the MALDI-TOF MS experiments, the proposed composition of the major lipid-A species identified are shown in Table 1. The remaining ions of lesser intensity, some of which represent lipid A where the 27-OHC28:0 modification has been replaced with 29-OHC30:0, are due to variation in the chain length of the fatty-acyl substituents.