Table 1.
OTA/CIT content in wheat purified on immunoaffinity column (IAC) [35,36] or by liquid partition [4], expressed in µg/kg (ppb).
| Samples | OTA ppb (c) | CIT ppb | |||
|---|---|---|---|---|---|
| IAC a/bicarbonate [35] | IAC b/PBS [36] | Liquid-Liquid/acid c [4] | Liquid-Liquid/acid d [4] | ELISA e | |
| F1 | <LOD | trace | 5.58 ± 0.28 | <LOD | <LOD |
| F2 | <LOD | trace | 6.45 ± 0.33 | <LOD | <LOD |
| F4 | <LOD | <LOD | 3.14 ± 0.15 | <LOD | <LOD |
| F6 | <LOD | <LOD | 0.84 ± 0.10 | <LOD | <LOD |
| F7 | <LOD | <LOD | 0.91 ± 0.11 | <LOD | <LOD |
| F8 | <LOD | <LOD | <LOD | <LOD | NA |
| F9 | <LOD | <LOD | Trace (<LOQ) | trace | NA |
| F10 | 128.49 ± 30.10 | 74.40 ± 8.20 | 6.26 ± 0.31 | 512.38 ± 61.5 | 1468.8 |
| F11 | <LOD | <LOD | <LOD | 0.84 ± 0.10 | <LOD |
| F12 | <LOD | <LOD | <LOD | <LOD | NA |
| F13 | <LOD | <LOD | <LOD | <LOD | NA |
| F14 | <LOD | <LOD | 1.07 ± 0.06 | <LOD | <LOD |
| F15 | <LOD | 1.5 ± 1 | 6.73 ± 0.35 | 0.86 ± 0.09 | <LOD |
| F16 | <LOD | <LOD | <LOD | 0.86 ± 0.1 | <LOD |
| F17 | <LOD | <LOD | <LOD | 0.76 ± 0.1 | <LOD |
| F18 | 67.23 ± 8.04 | 67.50 ± 9.50 | 30.77 ± 1.54 | 24.57 ± 2.7 | NA |
| F19 | <LOD | 3.20 ± 0.50 | <LOD | 7.24 ± 0.58 | NA |
| F20 | <LOD | trace | 5.20 ± 0.27 | <LOD | NA |
| S2 | 6.63 ± 2.33 | 5.07 ± 0.60 | 1.74 ± 0.09 | 44.60 ± 4.90 | 151.5 |
| S3 | <LOD | <LOD | <LOD | <LOD | NA |
| S4 | <LOD | <LOD | <LOD | 0.86 ± 0.10 | NA |
| S5 | <LOD | <LOD | <LOD | <LOD | NA |
| S6 | <LOD | <LOD | <LOD | 0.88 ± 0.09 | NA |
| S7 | <LOD | <LOD | <LOD | <LOD | NA |
| S8 | <LOD | <LOD | <LOD | 1.10 ± 0.09 | NA |
| S9 | <LOD | 3.06 | <LOD | 7.46 ± 0.89 | <LOD |
| S10 | <LOD | <LOD | <LOD | 1.04 ± 0.09 | <LOD |
| S11 | <LOD | <LOD | 5.3 ± 0.27 | <LOD | <LOD |
| S12 | <LOD | <LOD | 2.42 ± 0.3 | 1.57 ± 0.126 | <LOD |
| S13 | <LOD | <LOD | <LOD | 1.04 ± 0.09 | <LOD |
| S14 | 27.96 ± 3.01 | 25.20 ± 2.50 | 7.59 ± 0.38 | 3.79 ± 0.30 | <LOD |
| S15 | <LOD | <LOD | <LOD | <LOD | NA |
| S16 | <LOD | 1.00 ± 1.00 | <LOD | 15.00 ± 1.80 | 41.8 |