Table 1. Hybridoma formation from spleens of mice immunized with γDPGA in combination with CD40 agonist mAb.

Fusion no.*	Immunization protocol	IgM-secreting wells/total wells†	IgG-secreting wells/total wells†	Cell lines produced and cloned by limiting dilution
21	γDPGA + CD40 mAb (IP)‡	Not tested	1/192	21BL (IgG1)
25	γDPGA + CD40 mAb (IP)§	0/192	0/192	None
24	γDPGA + CD40 mAb (IP) + PGA IV boost¶	157/192	184/192	F24G7 (IgG3), F24F2 (IgG3)
26	γDPGA + CD40 mAb (IP) + PGA IV boost∥	33/192	20/192	F26G4 (IgG3), F26G3 (IgG3)

^*Each fusion represents a mouse immunized by use of the indicated immunization protocol.

^†After fusion, cells were distributed into 192 wells. The results indicate the number of wells containing colonies that secrete anti-γDPGA IgM or IgG.

^‡Spleens were harvested for hybridoma production 8 days after immunization with γDPGA plus CD40 mAb.

^§Spleens were harvested 29 days after immunization with γDPGA plus CD40 mAb.

^¶Mice were given an i.v. booster immunization with 0.5 μg of γDPGA 25 days after the initial immunization with PGA plus CD40 mAb; spleens were harvested 4 days later.

^∥Mice were given an i.v. booster immunization with 1.0 μg of γDPGA 17 days after the initial immunization with PGA plus CD40 mAb; spleens were harvested 4 days later.