Table 4.
Percentage methylation summary for Avy and CabpIAP loci.
| Exposure (ppm) | All live pups; % (SD) | All male; % (SD) | All female; % (SD) | a/a pups; % (SD) | a/a males; % (SD) | a/a females; % (SD) | Avy/a pups; % (SD) | Avy/a males; % (SD) | Avy/a females; % (SD) |
|---|---|---|---|---|---|---|---|---|---|
| CabpIAP (Avy/a and a/a offspring) mean methylation across four CpG sites | |||||||||
| 0 | 82.2 (6.7) | 82.4 (7.1) | 82 (6.3) | 82.7 (5.4) | 84.2 (4.8) | 80.6 (5.7) | 81.7 (7.7) | 80 (8.9) | 82.9 (6.7) |
| 2.1 | 83.5 (5.7) | 82.8 (6.5) | 84.3 (4.3) | 83.9 (6.7) | 83.3 (8.1) | 84.7 (4.6) | 83 (4.5) | 82.3 (4.7) | 83.9 (4.1) |
| 16 | 80.1 (9.4) | 79.7 (10.3) | 80.9 (7.6) | 79.2 (7.6) | 79.6 (7.7) | 78.8 (7.6) | 80.7 (10.7) | 79.7 (11.6) | 83.7 (6.9) |
| 32 | 82.7 (4.6) | 83.2 (4.8) | 82 (4.4) | 81.2 (4.2) | 81.4 (4.5) | 80.9 (3.9) | 83.9 (4.7) | 84.5 (4.6) | 83 (4.8) |
| Cubic trend p-value | 0.01* | 0.05* | 0.05* | 0.01* | 0.26 | 0.01* | 0.11 | 0.12 | 0.96 |
| Avy (Avy/a offspring) mean methylation across four CpG sites | |||||||||
| 0 | – | – | – | – | – | – | 29.5 (24.8) | 24.1 (26.1) | 33 (23.9) |
| 2.1 | – | – | – | – | – | – | 40.4 (24.8) | 41.3 (21.2) | 39.2 (29.4) |
| 16 | – | – | – | – | – | – | 27.1 (23.1) | 24.8 (23.1) | 34 (23.1) |
| 32 | – | – | – | – | – | – | 26.9 (21.8) | 29.4 (24.1) | 23.4 (18.5) |
| Cubic trend p-value | – | – | – | – | – | – | 0.04* | 0.02* | 0.85 |
*
p < 0.05 for the cubic trend.
a/a: Wild-type; Avy: Viable yellow agouti; CabpIAP: CDK5 activator-binding protein intracisternal A particle element; SD: Standard deviation.