Table 5.
Percentage methylation summary for Igf2 and Igf2r imprinted loci.
| Exposure (ppm) | All live pups; % (SD) | All male; % (SD) | All female; % (SD) | a/a pups; % (SD) | a/a males; % (SD) | a/a females; % (SD) | Avy/a pups; % (SD) | Avy/a males; % (SD) | Avy/a females; % (SD) |
|---|---|---|---|---|---|---|---|---|---|
| Igf2 (Avy/a and a/a offspring) mean methylation across eight CpG sites | |||||||||
| 0 | 39.3 (6.9) | 38.9 (7.4) | 39.7 (6.3) | 40.6 (7.1) | 40.9 (7.5) | 40.2 (6.5) | 37.9 (6.5) | 36 (6.4) | 39.3 (6.3) |
| 2.1 | 39.8 (9.8) | 40.4 (10.0) | 39.1 (9.6) | 41.1 (9.0) | 42.9 (8.1) | 38.9 (9.8) | 38.6 (10.5) | 38.1 (11.2) | 39.2 (9.8) |
| 16 | 39.2 (5.9) | 39.1 (6.5) | 39.3 (4.8) | 39.6 (4.7) | 38.4 (4.5) | 40.9 (4.6) | 38.9 (6.8) | 39.6 (7.5) | 36.8 (4.0) |
| 32 | 39.2 (5.1) | 39.6 (5.7) | 38.6 (4.2) | 39.9 (4.8) | 40.3 (5.5) | 39.4 (3.7) | 38.6 (5.4) | 39.1 (6.0) | 38.1 (4.5) |
| Cubic trend p-value | 0.51 | 0.37 | 0.76 | 0.50 | 0.07* | 0.38 | 0.97 | 0.85 | 0.45 |
| Igf2r (Avy/a and a/a offspring) mean methylation across five CpG sites | |||||||||
| 0 | 48.0 (5.1) | 48.2 (5.9) | 47.9 (4.0) | 49.1 (4.2) | 49.2 (5.0) | 49 (2.8) | 46.8 (5.9) | 46.8 (7.1) | 46.7 (4.9) |
| 2.1 | 49.4 (4.5) | 49.1 (5.3) | 49.6 (3.8) | 49.9 (3.7) | 52.6 (NA*) | 49.4 (3.9) | 49.2 (4.8) | 48.8 (5.4) | 49.8 (4.0) |
| 16 | 51.0 (4.3) | 51.7 (4.5) | 49.2 (3.3) | 50.9 (4.8) | 52.1 (5.1) | 48.9 (4.2) | 51.1 (4.1) | 51.6 (4.4) | 49.5 (2.6) |
| 32 | 49.6 (4.4) | 48.9 (2.5) | 50.3 (5.6) | 50.2 (3.4) | 48.2 (2.4) | 52.2 (3.1) | 49.2 (5.0) | 49.3 (2.6) | 49.1 (6.6) |
| Cubic trend p-value | 0.62 | 0.19 | 0.50 | 0.80 | 0.96 | 0.52 | 0.59 | 0.37 | 0.56 |
*
p < 0.10 for the cubic trend.
a/a: Wild-type; Avy: Viable yellow agouti; CabpIAP: CDK5 activator-binding protein intracisternal A particle element; NA: Not applicable; SD: Standard deviation.