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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Nov 2;1829(12):10.1016/j.bbagrm.2013.10.008. doi: 10.1016/j.bbagrm.2013.10.008

FIGURE 6.

FIGURE 6

TM4 cell characteristics. A. Quantitative measurement of Bcrp1 first exon expression in TM4 cells, relative to the expression of β-actin, using qPCR. B. Reporter assay of the activity of the E1U, E1A, E1B, and E1C promoters in TM4 cells. The data shown represent the mean and standard deviation of 3 different experiments, done on different days. Each individual assay was run in duplicate. The * indicate a significant difference from empty pGL3 vector control using the t-test (P<0.01). C. Expression of SF-1 mRNA relative to that of β-actin in C57BL6/J mouse testis and kidney, and in TM4 cells; Inset: Western blot showing SF-1 protein expression in mouse testis (C57BL6/J) and in TM4 cells. Results are typical of those obtained in 3 determinations, done on different days.