Figure 2. Significantly increased activation of Ras and its downstream targets with elevated COX2 and F4/80+ macrophages.
(A) Ras activity was measured by a Raf-pull down assay and was correlated with Phospho-ERK expression levels. (B) Western blot analysis for phospho-ERK expression for BAC and LSL/BAC animals on both diets was performed. (C) Additionally, IHC for phospho-ERK from all groups of mice are shown. (D & E) Pancreas tissue sections from each mouse were stained for COX2 and F4/80.