Table 2.
Summary of Pseudo-nitzschia ITS1 sequences used for in silico development of Pseudo-nitzschia-specific PCR primers and predicted length (including primer sequence) of the resulting ITS1 PnAll fragments.
| Species/typea | Predicted ARISA length (bp) |
No. sequences | Origin | GenBank accession no. |
|---|---|---|---|---|
| P. pungens type 1 | 142 | 1 | China | AY544769b |
| 1 | Mexico | AY257846c | ||
| 1 | Portugal | AY257845c | ||
| 1 | Vietnam | DQ166533b | ||
| P. pungens type 2 | 143 | 1 | Vietnam | DQ062665 |
| P. multiseries | 144 | 1 | Monterey Bay, California | AY257844c |
| 1 | Chesapeake Bay | DQ445651b | ||
| 1 | Ofunato Bay, Japan | DQ062664 | ||
| P. australis | 150 | 2 | Scotland | AY452527–528 |
| 1 | Monterey Bay | AY559850b | ||
| 1 | Portugal | AY257842c | ||
| P. seriata type 1 | 151 | 2 | Scotland | AY452523c–24c |
| 3 | Denmark | AY257841c, DQ062666c, DQ062663c | ||
| P. seriata f. obtusa | 147 | 1 | Tromsø, Norway | DQ062667c |
| P. delicatissima type 1 | 222 | 40 | Gulf of Naples, Italy | AY519282, AY519288–296, AY519302, AY519304, AY519305–06, AY519308, AY519310–11, AY519314, AY519316, AY519320, AY519322, AY519325–26, AY519328, AY519331–3, AY519334c, AY519335c, AY519336c, AY519338–347 |
| 1 | Boca Piccola, Italy | DQ336150c | ||
| 1 | Casttellamare, Italy | DQ329210c | ||
| P. delicatissima type 2 | 220 | 3 | Gulf of Naples, Italy | AY519303, AY519307, AY519337c |
| P. delicatissima type 3 | 223 | 8 | Gulf of Naples, Italy | AY519285–286, AY519299, AY519309, AY519315, AY519317, AY519323, AY519330 |
| P. delicatissima type 4 | 221 | 10 | Gulf of Naples | AY519287, AY519297, AY519300, AY519312–313, AY519318–319, AY519324, AY519327, AY519329 |
| P. delicatissima type 5 | 219 | 2 | Gulf of Naples | AY519301, AY519321 |
| P. delicatissima type 6 | 231 | 1 | Gulf of Naples | AY519283c |
| P. delicatissima type 7 | 165 | 2 | Gulf of Naples | AY519280–281 |
| P. delicatissima type 8 | 164 | 1 | Gulf of Naples | AY519279 |
| P. delicatissima type 9 | 210 | 1 | Gulf of Naples | AY519274 |
| P. delicatissima type 10 | 207 | 1 | Tasmania | AY257848c |
| P. delicatissima type 11 | 168 | 2 | Denmark | AY257849c, DQ329206c |
| 1 | Portugal | DQ329207c | ||
| P. delicatissima type 12 | 192 | 1 | Tuxpam, Mexico | DQ329211c |
| P. delicatissima type 13 | 212 | 1 | Ofunato Bay, Japan | DQ329208c |
| P. cuspidata type 1 | 230 | 1 | Canary Islands | AY257853c |
| 1 | Australia | AY257862c | ||
| P. cuspidata type 2 | 216 | 1 | Mexico | AY257852c |
| P. galaxiae type 1 | 151 | 1 | Mexico | AY257850c |
| P. galaxiae type 2 | 143 | 1 | Sydney, Australia | DQ336158c |
| P. pseudodelicatissima | 230 | 1 | Portugal | AY257854c |
| P. subpacifica | 195 | 1 | Limens, Spain | AY257859c |
| 1 | Costa Nova, Portugal | AY257858c | ||
| P. micropora type 1 | 146 | 1 | Vietnam | AY257847c |
| P. micropora type 2 | 143 | 1 | Phuket, Thailand | DQ329209c |
| P. inflatula | 156 | 1 | Phuket, Thailand | DQ329204c |
| P. spp. | 226 | 1 | Tasmania | AY257851c |
No symbol represents unknown identification method, but sequence is associated with published research.
ITS, internal transcribed spacer.
a
When multiple PnAll fragment lengths were predicted for a single species, each unique length was designated as a type (e.g., type 1, type 2, etc.).
b
Unknown identification method due to unpublished research.
c
Organisms identified using EM based on published research.