Figure 6. Metabolism of a 1:1 mixture of 1-InsP7 and 5-InsP7 by DIPP1.
1-InsP7 plus 5-InsP7 (10 nM each) were together incubated for 10 min with 0.3 ng DIPP1 plus approx 1000 D.P.M. of either 1-[3H]InsP7 (left hand bar) or 5-[3H]InsP7 (right-hand bar). HPLC analysis determined the metabolism of each InsP7. *p=0.02 (t-test; n=3).