Table 1. Ddp1p/DIPP kinetic data.
1-InsP7 | 5-InsP7 | InsP8 | ||||
---|---|---|---|---|---|---|
Km (nM) | 102 × kcat (s-1) | Km (nM) | 102 × kcat (s-1) | Km (nM) | 102 × kcat (s-1) | |
Ddp1p | 105 | 2.4 ± 0.05 | 93 | 0.4 ± 0.1 | 148 | 0.5 ± 0.1 |
DIPP1 | 42 | 110 ± 30 | 52 | 13 ± 3 | 85 | 10 ± 2 |
DIPP2α | 60 | 5 ± 2 | 35 | 0.7 ± 0.2 | 55 | 0.24 ± 0.08 |
DIPP2β | 70 | 1.7 ± 0.5 | 40 | 0.3 ± 0.09 | 42 | 0.16 ± 0.02 |
DIPP3α | 104 | 23 ± 2 | 146 | 4 ± 2 | 126 | 2.2 ± 0.4 |
DIPP3β | 73 | 8 ± 2 | 63 | 0.88 ± 0.07 | 78 | 0.37 ± 0.06 |
Kinetic data were obtained as described in Section 2. Average (n=3-5) substrate affinities were first compiled as -log Km (SEMs were <3% of the mean) and then transformed to Km for the Table.