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. Author manuscript; available in PMC: 2014 Dec 31.
Published in final edited form as: J Immunol Methods. 2013 Nov 5;0:10.1016/j.jim.2013.10.010. doi: 10.1016/j.jim.2013.10.010

Figure 1.

Figure 1

Depletion of T regulatory cells is accompanied by increased inflammation after intratracheal DTx administration in Foxp3DTR mice.

Foxp3DTR and C57Bl/6 mice were given three consecutive daily doses of 100ug Grade V OVA to induce inhaled tolerance. Ten days after the last dose of OVA, control PBS or DTx were given i.t. to tolerized mice. A) Representative flow cytometry gating strategy for identification of Tregs. After scatter gating, CD3+CD4+ T cells were identified. The gating of Foxp3+ cells is shown in the bottom left panel; CD25 expression on gated CD3+CD4+Foxp3+ cells is shown in the bottom right panel. B) BAL cell recovery in tolerized C57Bl/6 mice that received control PBS or DTx. Data are 48hr post treatment. C–D) Organ cell recovery (circles) and number of CD3+CD4+Foxp3+ cells (squares) in Foxp3DTR mice are reported for BAL (A) and MLN (B) each of the first four days after DTx administration. For organ cell count data, n=3–4 per group per day. For Foxp3+ cell quantification, all are n=3–4 per group per day, but Control group was pooled. Percentages in A) are percent reduction from Control group. * = p<0.05 compared to Control.