Fig. 3.

Immunohistochemistry staining for galanin in WT dispersed primary hippocampal neurons and slice organotypic cultures. (A) One-half of all dispersed neurons are positive for galanin after 9 days in culture (Upper Left), corresponding bright-field image (Upper Right), and overlay (Lower). (B) High levels of galanin staining are noted throughout WT organotypic cultures. Because of the thickness of the slice culture (≈250 μm after 2 wk in culture), the neurons in the periphery of the field are not in focus.