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. 2004 Mar 23;101(14):5105–5110. doi: 10.1073/pnas.0304823101

Fig. 4.

Fig. 4.

Responses of hippocampal cultures after incubation with 10 nM to 1 μM staurosporine (St). (A) Organotypic cultures from galanin knockout (KO) cultures had significantly more neuronal cell death than WT controls at each concentration of staurosporine in the CA1 subfield. Cell death was visualized by propidium iodide staining. Results are expressed as a percentage of the area expressing fluorescence as compared to the untreated “control” cultures. Each point represents the mean ± SEM, n = 4. **, P < 0.01; ***, P < 0.001. (B) Primary hippocampal cultures were incubated for with 10 nM to 1 μM staurosporine. KO cultures had significantly more neuronal cell death than WT cultures at each concentration of staurosporine. Each point represents the mean ± SEM. n = 4. **, P < 0.01; ***, P < 0.001. (Bar = 100 μm.) (C) Organotypic cultures from galanin overexpressing (OE) mice were incubated with 50 nM to 1 μM staurosporine. OE cultures had significantly less neuronal cell death than WT cultures at 100 nM and 50 nM staurosporine. Each point represents the mean ± SEM. n = 4, **, P < 0.01, ***, P < 0.001.