Figure 5.

Effect of purine-modified ADPR analogues on activation of TRPM2 by 1. Maximum outward currents were recorded as outlined in the Experimental Section. Pipet solutions contained either no nucleotide or 100 μM ADPR (1) alone or in combination with the indicated ADPR analogue. 8-Br-ADPR (4) was included as inhibitor control. Bars are grouped to indicate the position of purine modification (label on top). A horizontal dashed line marks the mean of the control (100 μM of 1). Data are shown as mean ± SEM (number of experiments denoted in or on top of the bar) and were analyzed by a nonparametric one-way ANOVA (Kruskal–Wallis test) followed by comparison against control (100 μM of 1) using Dunn’s correction for multiple testing. Results significantly different from control (p < 0.05) are indicated by asterisks.