Figure 7.

Effect of pyrophosphate-modified ADPR analogues on TRPM2 activation by ADPR (1) Maximum outward currents were recorded as outlined in the Experimental Section. Pipet solutions contained either no nucleotide or 100 μM of 1 alone or in combination with the indicated ADPR analogue. (A) Analogues that were soluble in buffer. (B) Analogues that were only soluble on addition of DMSO. Because squaryl and triazole compounds (64–67, 73) were resolubilized in DMSO, the pipet solution contained 0.1% DMSO. For the control conditions, DMSO was included in the pipet solution at the same final concentration. Data are shown as mean ± SEM (number of experiments denoted in or on top of the bar) and were analyzed by a nonparametric one-way ANOVA (Kruskal–Wallis test) followed by comparison against the respective control (100 μM of 1) using Dunn’s correction for multiple testing. Results significantly different from control (p < 0.05) are indicated by asterisks.