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. Author manuscript; available in PMC: 2014 Oct 29.
Published in final edited form as: Biochemistry. 2013 Oct 18;52(43):10.1021/bi401034q. doi: 10.1021/bi401034q

Figure 1.

Figure 1

C-furSema potently inhibits VEGF-A mediated VEGFR2 activation in situ. A) A schematic representation of Sema3 highlighting the furin-processed C-terminal domain (C-furSema). B) Activation of PAE cells stably overexpressing Nrp1 and VEGFR2 by VEGF-A is inhibited by C-furSema as demonstrated by a decrease in VEGFR-2 Tyr-1175 auto-phosphorylation. C) C-furSema is a potent dose-dependent inhibitor of VEGF-A mediated HUVEC activation as demonstrated by sandwich ELISA (IC50 = 34 ± 15 nM). Phospho-VEGFR-2 levels were normalized to VEGF-A alone and 1 μM sunitinib.