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Effect of the intermolecular disulfide on C-furSema potency. A) Comparison of dimeric C-furSema and C-furSemaMon B) reveals a critical role for the conserved inter-molecular disulfide C-furSema (grey line, IC50 = 24 ± 1 nM) and C-furSemaMon (black line, IC50 = 1.3 ± 0.7 μM) in inhibiting VEGF-A binding to Nrp1.
