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. Author manuscript; available in PMC: 2014 Oct 29.
Published in final edited form as: Biochemistry. 2013 Oct 18;52(43):10.1021/bi401034q. doi: 10.1021/bi401034q

Figure 5.

Figure 5

The helical motif determines potent competitive Nrp1 binding. A) To determine the contribution of N-terminal helical region in mediating Nrp inhibition, C-furSemaHet was synthesized. B) Comparison of non-reducing versus reducing SDS-PAGE demonstrates the purity and correct intermolecular disulfide bond formation of C-furSema and C-furSemaHet. C) C-furSema and C-furSemaHet equivalently inhibit VEGF-A dependent activation of PAE cells and D) have nearly equal potencies, IC50 = 24 ± 1 nM vs. IC50 = 35 ± 3 nM, respectively.