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View full-text article in PMC

. Author manuscript; available in PMC: 2013 Dec 27.

Published in final edited form as: Nat Commun. 2013;4:1973. doi: 10.1038/ncomms2973

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(A) The figures illustrate DNA-protein binding patterns analyzed by electrophoretic mobility shift assay. Biotin-labeled double stranded probes were incubated with nuclear extract from HEK293 cells. (Left) A diplotype probe carrying both rs806370 and rs806371 was compared to wild type (WT) probe. (Right) DNA-protein binding patterns were compared between WT probe, and single variant probes carrying only rs806370 or rs806371. The additional shift bands are indicated by arrows. (B) The figure illustrates normalized fold change for CNR1 promoter activity in vitro. Reporter gene constructs, with or without rs806371, were transient transfected into Huh-7 cells. Luciferase activities were measured at 48h, 72h and 96h. Results represent mean ± S.E. for experiments run in triplicate.

(A) The figures illustrate DNA-protein binding patterns analyzed by electrophoretic mobility shift assay. Biotin-labeled double stranded probes were incubated with nuclear extract from HEK293 cells. (Left) A diplotype probe carrying both rs806370 and rs806371 was compared to wild type (WT) probe. (Right) DNA-protein binding patterns were compared between WT probe, and single variant probes carrying only rs806370 or rs806371. The additional shift bands are indicated by arrows. (B) The figure illustrates normalized fold change for CNR1 promoter activity in vitro. Reporter gene constructs, with or without rs806371, were transient transfected into Huh-7 cells. Luciferase activities were measured at 48h, 72h and 96h. Results represent mean ± S.E. for experiments run in triplicate.