TABLE 1:
Average filament elongation rate.
| Barbed ends | Tether | Actin (μM) | Mg2+ (mM) | Seeds (nM) | Profilin (μM) | Barbed-end rate (subunits/s) | Number of filaments | Rate of enhancement |
|---|---|---|---|---|---|---|---|---|
| Tethered | PP-WWCA | 1.2 | 1 | — | — | 22.42 ± 0.39 | 10 | 3.25 |
| Free | PP-WWCA | 1.2 | 1 | — | — | 6.89 ± 0.13 | 10 | |
| Tethered | PP-WWCA | 1 | 10 | — | — | 21.05 ± 0.39 | 10 | 3.44 |
| Free | PP-WWCA | 1 | 10 | — | — | 6.12 ± 0.08 | 10 | |
| Tethered | PP-WWCA | 1 | 10 | 75 | 1 | 14.28 ± 0.18 | 7 | 1.93 |
| Free | PP-WWCA | 1 | 10 | 75 | 1 | 7.40 ± 0.11 | 13 | |
| Tethered | WWCA | 1 | 1 | — | — | 21.45 ± 0.52 | 10 | 3.14 |
| Free | WWCA | 1 | 1 | — | — | 6.83 ± 0.09 | 10 | |
| Tethered | WWCA | 1 | 10 | — | — | 21.74 ± 0.70 | 10 | 3.45 |
| Free | WWCA | 1 | 10 | — | — | 6.29 ± 0.11 | 10 | |
| Tethered | WWCA | 1 | 10 | 75 | 1 | 25.45 ± 1.09 | 10 | 3.45 |
| Free | WWCA | 1 | 10 | 75 | 1 | 7.37 ± 0.28 | 10 |