Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 1;25(1):66–75. doi: 10.1091/mbc.E13-04-0200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Clayton et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 4: — Estimating the numbers of Myo52p molecules per motile particle in the cell. The signal intensity of GFP in motile Myo52p-3xGFP particles was used to estimate the number of Myo52p molecules/particle. Clathrin light-chain (Clc1p-3xGFP) signal was used as a standard based on its previously characterized distribution at actin patches (40 molecules/patch; Sirotkin et al., 2010). (A) Representative time-lapse images of a mixed population of myo52-3xGFP and clc1-3xGFP cells (after growth on YE5S rich medium at 25°C). clc1-3xGFP cells were distinguished from myo52-3xGFP cells by inclusion of a genomic uch2-Cherry fusion, which marks nuclei red. The GFP (green) images shown are maximum projections generated from seven z-stacks. The arrowheads track the movement of three different motile Myo52p particles. Bar, 4 µm. (B) Histogram comparing the average GFP intensities for motile Myo52p particles and Clc1p patches. (C) Histogram of the distribution of Myo52p GFP intensities expressed as number of dimeric motors/motile particle.