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. 2014 Jan 1;25(1):95–106. doi: 10.1091/mbc.E13-07-0376

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© 2014 Mazel et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Correlation of dynein speckle dynamics with short-microtubule behavior. (A) Sequence showing a short microtubule, which pivots around a dynein speckle. At the first encounter with a dynein speckle, the short microtubule moves only marginally. After this brief delay, the short microtubule swivels around the dynein speckle (white arrows) while moving forward. At a later time point, a visible speckle (white arrows) accumulates at the trailing end of the same short microtubule. The disappearance of this speckle correlates with microtubule detachment from the cell cortex. (B) Sequence of a short microtubule that forms a transient kink during sliding. The short microtubule slides directionally and forms a kink at the position of a bright and persistent speckle (white arrows). This kink is lost from one frame to the other, simultaneous with the loss of this bright speckle (see also enlarged inset). See also Supplemental Figure S8 and Supplemental Movies S9 and S10.