TABLE 2:
Parameters for stochastic simulation of short microtubules.
| Parameter | Simulation shown in Figure 5, A and Ba | Simulations shown in Figure 5Ca | Units | Reference |
|---|---|---|---|---|
| Number of nucleated short microtubules | 262 | 50 | This work | |
| Number of dynein complexes | 434 | Varied with density | This work | |
| Dynein complex density | 0.16 | 0.03125–2 | μm−2 | This work |
| Microtubule length (mean ± SD) | 5.0 ± 0.7 | 5.0 ± 0.7 | μm | This work |
| Dynein speckle turnover rate | 0.5 | 0.005–50 | s−1 | This work |
| Directional microtubule velocity (based on maximal dynein speed) | 48 | 48 | μm/min | Toba et al. (2006) |
| Dynein reach distance | 82.5 | 82.5 | nm | Gennerich et al. (2007) |
| Effective diffusion coefficient for short-microtubule fluctuations at the cell cortex (corresponding to temperature of 35°C and dynamic viscosity of 0.05 Pa⋅s) | 0.016 | 0.016 | μm2/s | Modified from Nedelec (2002) |
| Simulation time step | 0.01 | 0.01 | s |
aValues from this work derived from the cell shown in Figure 5.