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. 2004 Mar 26;101(14):5164–5169. doi: 10.1073/pnas.0300084101

Fig. 4.

Fig. 4.

Characterization of protein phosphatase activity encoded by a GST-DIG3 fusion protein. (A) The DIG3 protein is a serine/threonine phosphatase. One microgram of GST or GST-DIG3 was incubated with: a, No substrate; b, 100 μM substrate; c, 100 μM substrate plus 1 mM sodium vanadate; d, 100 μM substrate plus 50 mM sodium fluoride. □, GST; ▪, GST-DIG3. (B) The DIG3 protein is a PP2C. One microgram of GST or GST-DIG3 was incubated with or without 100 μM substrate in each of the three buffers specific for PP2A, PP2B, or PP2C activity. The data are average values of three experiments. Error bars represent standard deviations.