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. 2013 Dec 27;8(12):e81423. doi: 10.1371/journal.pone.0081423

Figure 1. The expression of IGFBP3 is regulated by HoxD10 in human gastric cancer cells.

Figure 1

The expression of IGFBP3 was detected by conventional RT-PCR (A, C) and Western blotting (B, D) after transfected with pcDNA3.1 empty vector or pcDNA3.1-HoxD10 in BGC823 and SGC7901 cells, and negative control siRNA (Ne-Si) or two of HoxD10 siRNA (H-Si1 and H-Si2) in HGC27 cells. GAPDH was used as internal control. Densitometry values are expressed as fold change compared with pCDNA3.1 vector or negative control siRNA values normalized to 1. (E) BGC823 and SGC7901 were co-transfected with pcDNA3.1 empty vector or pcDNA3.1-HoxD10, pGL3-basic vector or pGL3-(pIGFBP3)-basic and pRL-TK vector. Relative firefly activity was expressed normalized to renilla activity in pRL-TK vector. All experiments were performed in triplicate. ** indicates of p<0.01.