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. 2013 Dec 27;8(12):e81423. doi: 10.1371/journal.pone.0081423

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© 2013 Xue et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of IGFBP3 promoter with the potential binding sites for HoxD10 (HBS1-5) and four fragments (A1-A4) spanning five HBSs at the IGFBP3 promoter region were designed for PCR analysis in ChIP assays (A1-A4). (B) HoxD10 overexpressed BGC823 and SGC7901 cells were cross-linked by formaldehyde and lysed. Cell lysates were subjected to immunoprecipitation with either a rabbit antibody to HoxD10 or normal rabbit IgG. Genomic DNA and 2% input DNA were used as positive control.