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. 2013 Dec 27;8(12):e81423. doi: 10.1371/journal.pone.0081423

Figure 3. Identification of the regulatory regions of IGFBP3 which are responsible by HoxD10 in BGC823 cells.

Figure 3

(A) BGC823 cells were transfected with pcDNA3.1 empty vector or pcDNA3.1-HoxD10, pGL3-promoter vector or pGL3-HBSI(II)-promoter and pRL-TK vector. (B) Different oligonucleotides which contain wild or point mutant sequences of HBS3, HBS4 and HBS5 were cloned into pGL3-HBS-promoter. Relative firefly activity was expressed normalized to renilla activity in pRL-TK vector. All experiments were performed in triplicate. ** indicates of p<0.01.