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. 2013 Dec 27;8(12):e82777. doi: 10.1371/journal.pone.0082777

Figure 6. Metabolism contribution to E-[c(RGDfK)2] or iRGD internalizations: Effect of the cellular density.

Figure 6

U87 cells were incubated for 1-[c(RGDfK)2] (A) and iRGD (B). Incubations were performed for different media conditions: with Glucose and pyruvate, without oligomycin (condition 1); with Pyruvate, without Glucose and oligomycin (condition 2); without Glucose, pyruvate and oligomycin (condition 3); with Glucose, pyruvate and oligomycin (condition 4); with pyruvate and oligomycin, without Glucose (condition 5); with oligomycin, without Glucose and pyruvate (condition 6). All the experiments were performed at least 3 times. For the E-[c(RGDfK)2] the results for all the conditions (★,Inline graphic,☆,Inline graphic,Inline graphic,Inline graphic,▾,▵) were significantly different with p<0.01. For the iRGD the results for all the conditions (▪,Inline graphic,□,•,Inline graphic,○,♦,Inline graphic,⋄) were significantly different with p<0.01.