Table 3. Abundances of prokaryotes in Breitenbach streambed sediments experimentally rewetted after 13 weeks of desiccation.
treatment | days | Prokaryotes | Bacteria | Alphaproteobacteria | Betaproteobacteria | Gammaproteobacteria | Bacteroidetes | Actinobacteria | Firmicutes |
(109 cells mL−1) | (109 cells mL−1) | (107 cells mL−1) | (107 cells mL−1) | (107 cells mL−1) | (107 cells mL−1) | (107 cells mL−1) | (107 cells mL−1) | ||
dry | 0 | 4.1±2.3 | 1.7±0.2 | 18.6±13.6 | 17.5±2.2 | 1.0±1.7 | 1.9±0.5 | 18.1±7.0 | 3.9±2.1 |
with cells | 1 | 3.9±1.2 | 1.2±9.7 | 33.1±13.5 | 50.2±19.2* | 16.2±4.3* | 21.8±11.2 | 20.0±16.5 | 11.1±17.9 |
2 | 3.1±1.8 | 1.0±0.3 | 44.2±22.6 | 111.7±2.8** | 17.3±14.5 | 18.7±20.5 | 5.2±3.7* | 5.2±4.2 | |
3 | 3.6±0.9 | 1.9±2.1 | 49.7±24.9 | 128.7±102.2* | 13.8±9.4* | 42.4±30.0* | 8.3±5.7 | 3.5±2.0 | |
6 | 5.4±2.6 | 2.0±2.7 | 32.3±17.5 | 235.0±141.8 | 25.0±17.3 | 11.8±9.9 | 11.2±11.2 | 5.3±3.3 | |
10 | 10.7±5.6* | 2.7±2.8 | 107.2±18.5* | 115.6±133.8 | 8.9±8.7 | 16.8±7.3* | 12.8±4.7 | 8.9±6.7 | |
14 | 7.5±0.9* | 2.6±1.1 | 100.7±73.1 | 128.2±65.0* | 14.7±14.8 | 10.0±5.0 | 5.2±3.0 | 10.6±3.6* | |
without cells | 1 | 2.8±1.8 | 1.6±0.7 | 30.4±12.9 | 33.6±7.6* | 8.3±6.7 | 11.0±7.3 | 4.3±4.4 | 8.0±6.3* |
2 | 2.1±1.3 | 0.5±0.3 | 50.9±36.2 | 46.4±23.1 | 14.6±13.9 | 4.3±4.4 | 14.7±20.8 | 10.4±3.0* | |
3 | 3.6±2.1 | 1.3±9.6 | 59.4±59.3 | 190.5±111.2 | 67.2±23.3* | 11.1±11.6 | 7.9±1.3 | 13.1±4.7 | |
6 | 2.8±1.5 | 1.1±1.7 | 17.6±15.9 | 53.3±41.7 | 33.1±52.8 | 26.1±16.4* | 15.1±13.2 | 9.6±7.4 | |
10 | 6.4±1.8 | 0.8±0.7 | 28.0±21.2 | 45.1±13.0* | 47.8±41.9 | 12.0±7.3 | 5.3±2.6* | 5.7±4.0 | |
14 | 4.2±1.7 | 0.9±0.5 | 37.3±25.4 | 74.0±25.4* | 7.4±2.2* | 8.4±1.5* | 6.7±1.1* | 7.4±1.1 |
The abundance of prokaryotes was determined after SYBR Green staining, whereas the abundances of different taxonomic groups were determined via CARD-FISH. Means with SD are given (n = 4). The asterisks indicate significant differences between dry sediment from day 0 used for rewetting and the treatment samples (ANOVA, * = P<0.05, ** = P<0.01).