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. 2013 Dec 27;8(12):e83982. doi: 10.1371/journal.pone.0083982

Figure 1. Zip1 disappearance is delayed in ipl1-mn mutants.

Figure 1

(A) Experimental set up. SCs are followed by Zip1-GFP; SPBs by Cnm67-mCherry, and anaphase I onset by Pds1-tdTomato (confluent staining). (B) Representative montages of SC disassembly (loss of Zip1-GFP), SPB separation (CNM67-mCherry), and anaphase I onset (degradation of Pds1-tdTomato) in wild type (Movie S1) and ipl1-mn (Movie S2). Bars: 2 µm. (C) The proportion of cells with linear Zip1-GFP structures, diffuse Zip1-GFP staining or no Zip1-GFP signal at anaphase I onset (Pds1 degradation). (D) Time from SC disassembly (Zip1-GFP signal loss) to separation of the SPBs. (E) Time from SC disassembly to anaphase I onset (Pds1 degradation). Strains: WT, Y4044 and ipl1-mn, Y4047.