Skip to main content
. 2013 Dec 27;8(12):e83982. doi: 10.1371/journal.pone.0083982

Figure 7. S-CDK is required and sufficient to drive SPB separation and spindle formation during prophase I in ipl1-md cells.

Figure 7

(A)Images for tubulin and Zip1 staining in dmc1Δ ipl1-md strains with normal S-CDK and M-CDK (left image), lacking S-CDK activity (clb5Δ clb6Δ; middle image), or without M-CDK proficient for Clb5 only (clb1Δ, clb3Δ, clb4Δ, clb6Δ CLB5+; right panel). Strains: Y4495, Y4435, and Y4496, respectively. Bars, 2 µm. (B) Quantification on the proportion of fixed cells with spindles and separated SPBs at 8 hours and 12 hours. (C, D) ipl1-md ndt80Δ cdc28-as1 (Y2577) cells were treated with either 50 µM 1-NM-PP1 (+) or solvent only (DMSO) (−) to inhibit Cdc28/CDK kinase activity at 6 hours, when spindles have formed in at least 20% of ipl1-md ndt80Δ cells. Examples of spread, meiotic nuclei are shown to the left. Note that there was no effect on inhibiting Cdc28-as1 in ndt80Δ alone bars, 2 µm. The graph shows that Quantification of prophase spreads with spindles or aberrant spindle pole structures (e.g. multipolar spindles).