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. 2013 Dec 27;8(12):e84060. doi: 10.1371/journal.pone.0084060

Figure 2. Whi3 localizes to stress granules during heat shock.

Figure 2

(A) Cells co-expressing Pub1-mCh and Whi3-GFP were heat shocked at 46°C for 15 minutes. Heat shock caused Whi3 and Pub1 to colocalize in distinct cytoplasmic foci. When cells were returned to 30°C, these foci dissolved. Scale bar represents 5 µm. (B) Pelleting assay results support the observation that Whi3 is enriched in insoluble stress granules during heat shock. Both Whi3FL-3xGFP and Pub1-mCh are enriched in soluble (S) fractions at 30°C, but become enriched in the insoluble pellet (P) fraction during heat shock at 46°C. Housekeeping gene Pgk1 does not change solubility in response to stress. (C) Whi3ΔQrich-3xGFP colocalizes with stress granule marker Pub1-mCherry during heat shock. (D) Whi3ΔQrich exhibits a similar solubility profile to Whi3FL-3xGFP. (E) Whi3ΔRRM-3xGFP colocalizes with stress granule marker Pub1-mCherry during heat shock. (F) Whi3ΔRRM exhibits a similar solubility profile to Whi3FL-3xGFP.