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. 2013 Dec 27;8(12):e84366. doi: 10.1371/journal.pone.0084366

Figure 1. Smad4 knockdown and KRASG12V expression in the H6c7 cell line.

Figure 1

(A) A schema of introducing KRASG12V and Smad4 loss using the H6c7 cell line to produce the shSmad4, H6c7 KRAS shSmad4, TβR, TβR-KRAS , and TβR-KRAS -Smad4 cell lines. (B) Immunoblot of RAS-GTP, KRAS, and Smad4. GAPDH was used as a loading control. (C) PAI-1 mRNA expression after 48 hours of TGF-β stimulation (n=3). (D) Invasion assays through Matrigel coated membranes incubated with and without TGF-β (n=6). (* denotes significant differences between the test and control NS samples, and between treatment and no treatment groups two-way ANOVA and Bonferroni’s post hoc tests or paired student t-test; p<0.05; data are presented as mean ± SEM) .