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. 2013 Dec 27;8(12):e85065. doi: 10.1371/journal.pone.0085065

Figure 4. DegU32(Hy) and DegS200(Hy) behave differently in EMSA.

Figure 4

Fluorescently labeled PA(fla/che) was challenged with the mutant DegU32(Hy) or DegS200(Hy) proteins in EMSA. DegS200(Hy) was used to phosphorylated wild-type DegU in lanes 10 and 11. DegU32(Hy) was phosphorylated by wild-type DegS in lanes 5 and 6. Protein concentrations were the following: 0.2 μM for DegS; 0.4 μM for DegS200(Hy); 0.36 μM for DegU and 1 μM for SwrA. Different concentrations of DegU32(Hy) were used: 0.3 μM in lane 2; 0.6 μM in lanes 3, 5 and 6; 1.8 μM in lane 4. Control reactions with wild-type proteins are shown in lanes 7, 8, 9. An asterisk marks the particular pattern of bands, bracketed on the left-hand side of the gel, produced by DegU32(Hy) and DegU32(Hy)~P. Other symbols are the same as in Figure 2B.