Figure 5. Motility defects caused by the swrA ^- and degU32(Hy) alleles are suppressed by an optimized P_A(fla/che).

In the A and B panels swimming (left) and swarming (right) performances of swrA ^- or swrA ^- degU32(Hy) strains containing the dhsA6 mutation is respectively shown. The single nucleotide dhsA6 mutation in P_A(fla/che) improves similarity to the σ^A consensus sequence [16] and thus the predicted promoter strength. Motility of the parental swrA ^- and swrA ^- degU32(Hy) strains is shown in C and D respectively. Relevant genotypes are indicated on top of each panel. Strains used to generate the images are: PB5452 (A); PB5447 (B); PB5370 (C); PB5384 (D).