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. 2013 Dec 27;8(12):e84465. doi: 10.1371/journal.pone.0084465

Figure 3. Dose-dependency of AP20187.

Figure 3

(A) RAW264.7 and RAW264.7+iRANK cells were cultured in medium alone, or medium containing vehicle (EtOH), RANKL, or 0.1–50 nM AP20187 for 4 days and the cells were stained for TRAP. The number of TRAP-positive multinucleated cells (MuNC) was determined per 4 high power fields of view and averaged over 3 wells. Cells containing more than three nuclei were counted as multinucleated cells. (B) Dose-dependent induction of TRAP activity following RANKL or AP20187 treatment. Left panel: RAW264.7 cells. Right panel: RAW264.7+iRANK cells. The TRAP-activity in RAW264.7+iRANK cells reached a maximum at the 50 nM AP20187 treatment. #p<0.05 compared to medium, *p<0.05 compared to 0.1% ETOH.