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. 2013 Dec 27;8(12):e84465. doi: 10.1371/journal.pone.0084465

Figure 5. NF-κB signaling.

Figure 5

NF-κB dependent signaling in engineered osteoclasts. RAW264.7 and RAW264.7+iRANK cells were transiently transfected with a luciferase reporter construct containing NF-κB sites derived from Igκ promoter driving the luciferase gene and a Renilla luciferase construct as the internal control. NF-κB activation was measured in RAW264.7+iRANK cells stimulated with AP20187 (A), and RAW264.7 cells stimulated with RANKL (B) or LPS (C) for 2 and 4 h. Data are average relative light units (RLU) per µg protein +/− SD. *p<0.05.