Figure 6. Treatment with C2IN-C3lim decreases the resorption of synthetic calcium phosphate surfaces by already differentiated osteoclasts.

C2IN-C3lim (0.5 and 2µg/mL) was added to osteoclasts growing on synthetic calcium phosphate surfaces from day 5 on, i.e. after undisturbed osteoclast-differentiation from RAW264.7 cells. For control, no C2IN-C3lim was added. At day 13, resorption activity of osteoclasts was determined by quantification of the resorbed area per well (A). Qualitative images of resoption areas on sythetic calcium phosphate surfaces are shown below (B, left: control; right: C2IN-C3lim (2µg/mL)).