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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Mol Pharm. 2013 Aug 5;10(9):3296–3303. doi: 10.1021/mp300720k

Figure 4.

Figure 4

Representative brightfield and fluorescence micrographs showing dead/dying MDA-MB-231 cells after treatment with a binary mixture of nucleic acid stain SYTOX Blue (5 μM) and either Tetra-Zn2BDPA-SR (a) or Bis-Zn2BDPA-SR (b) (10 μM). In each case, the cells were initially treated with etoposide (15 μM) for 11 hr, incubated with the probes for 30 min at 37 °C, then washed with HEPES buffer. Scale bar = 30 μm. Healthy cells are unstained, cells only stained red are apoptotic, and cells stained both red and green are necrotic.