Table 2.
Various cargoes that have been loaded within, or conjugated to, DNA nanocarriers. Loading is given on a number per carrier basis. Abbreviations: nt,nucleotides; WF, wireframe; cyt c, cytochrome c; EGFP, enhanced green fluorescent protein; Fab’, antibody fragment; Dox, doxorubicin; Au NP, gold nanoparticle.
| Cargo Type | Size | Loading | Container | Cargo-Container Stabilization |
Citation | |
|---|---|---|---|---|---|---|
| Nucleic acid | ||||||
| CpG | 18 nt | 1-4 | WF Tetrahedron | Integrated | [35] | |
| CpG | 20 nt | up to 62 | Origami tube | Overhangs | [118] | |
| Antisense | 20 nt | 1 | WF Tetrahedron | Integrated | [5] | |
| Aptamer | 25 nt | 12 | WF Icosahedron | Integrated | [3] | |
| siRNA | 30 nt | 1-6 | WF Tetrahedron | Overhangs | [9] | |
| Protein | ||||||
| cyt c | 12.4 kDa | 1 | WF Tetrahedron | Covalent | [119] | |
| EGFP | 27 kDa | 1-4 | WF Tetrahedron | Covalent | [120] | |
| EGFP | 27 kDa | 1 | WF Tetrahedron | Non-covalent | [39] | |
| Fab’ | 55 kDa | 1-12 (avg 3) | Origami barrel | Overhangs | [37] | |
| Streptavidin | 60 kDa | 1-4 | WF Tetrahedron | Non-covalent | [40] | |
| IgG | 150 kDa | 1 | WF Tetrahedron | Non-covalent | [40] | |
| Drugs | ||||||
| Dox | 0.54 kDa | 1200 | WF Icosahedron | Intercalation | [3] | |
| Dox | 0.54 kDa | 1800* | Origami triangle & tube |
Intercalation | [8] | |
| Dyes | ||||||
| YOYO-1 | 1.3 kDa | 48 | WF Tetrahedron | Intercalation | [121] | |
| Inorganic | ||||||
| Au NP | 5 nm diam. |
1-12 (avg 4) | Origami barrel | Overhangs | [37] | |
| Au NP | 15 nm diam. |
many | WF tube | -- | [36] |
*
loading is estimated based on reference [3].