Table 1.
The effect of USSC and scaffold combination on the expansion of UCB-CD34+ over 3 weeks. Values are expressed as mean ± SD for 3 numbers of experiments. Fold expansion was calculated based on the cell counts (1000) on day 0
| Total Cells (fold exp.) | |||
|---|---|---|---|
| Groups | 1st wk | 2nd wk | 3rd wk |
| S2D (n=3) | 13800±1600 (13.8±1.6) |
53400±3200 (53.4±3.2) |
101900±8000 (101.9±8) |
| F2D (n=3) | 28700±3400 (28.7±3.4) |
104300±11000 (104.3±11) |
194200±4600 (194.2±4.6) |
| MBA (n=3) | 40800±4100 (40.8±4.1) |
145500±5800 (145.5±5.8) |
271700±13000 (271.7±13.2) |
| DBM (n=3) | 44200±1600 (44.2±1.6) |
176700±6400 (176.7±6.4) |
305800±12700 (305.8±12.7) |
Abbreviation: simple 2D culture (S2D), simple 2D culture with feeder (F2D), mineralized bone allograft (MBA), de-mineralized bone matrix (DBM)