TABLE 2.
C. albicans glycans and adhesion
| C. albicans strain [serotype] | Substratea (source)b | Growth mediumc (temp) | Assay format | Evidence for glycand | Reference |
|---|---|---|---|---|---|
| Clone 4 [B] | Fibrin-platelet matrix | Phytone peptone + Glc, 1 mg ml−1 | Radiolabeled fungi | Inhibition by α-mannosidase | 184 |
| Clinical isolate | Urothelial cells (h) | Tryptose phosphate | Microscopy | Ruthenium red staining | 188 |
| CBS562 (ATCC 18804) | VEC (h) | Yeast extract (28 or 37°C) | Mix and filter | Inhibition by chitin, GlcNAc, ManN, GlcN | 255 |
| MSU-1 | BEC (h) | TSB + 4% Glc; M199 (37°C) | Mix and filter | Inhibition by Con A (reversed by α-MM) | 245 |
| Clinical isolate | Urothelial cells, BEC (h) | SDA (37°C) | Mix and filter | Inhibition by Man | 47 |
| VW32 [A] | BEC (h) | SDA (37°C) | Mix and filter | Staining with Con A | 296 |
| MSU-1 | BEC (h) | TSB + 4% Glc; M199 (37°C) | Mix and filter | Inhibition by Con A, extraction of carbohydrates | 244 |
| 20A | Liver (m) | TSB + 0.5 M Gal, RPMI 1640 + Glc (24 or 37°C) | Perfusion and flow (elution) | Inhibition of trapping by Man, α-MM | 250 |
| M1012R AL mannan mutants, clinical isolates [A and B] | BEC (h) | SDB (27°C) | Mix and filter | Decreased adhesion in absence of β-1,2-oligo- mannosides | 195 |
| A9 [B] | Splenic and lymph node sections (m) | GYEP (37°C) | Microscopy | Inhibition by periodate oxidation or α-mannosidase | 146 |
| A9 [B], Strain 1 [A], 222 [B] | Splenic sections (m) | GYEP (37°C) | Microscopy | Inhibition by monoclonal antibody 10G or its antigen (β-1,2-Man4) | 179 |
| A9 [B], TCH32 [A], NUM1385 [A] | Splenic sections (m) | GYEP (37°C) | Microscopy | Periodate oxidation prevents binding activity | 144 |
| A9 [B], S. cerevisiae x2180, S. cerevisiae MNN mutants | Splenic sections (m) | GYEP (37°C) | Microscopy | Inhibition by α-mannosidase | 145 |
| SC5314 [A], CAI4, NGY21, NGY23, NGY24 | Vaginitis (r), BEC (m or gp) | Specific medium not indicated | Colony counts | Decreased adhesion in absence of O-linked glycans | 27 |
| ATCC 10231, ATCC 10261 | GI epithelium (m) | SDA (37°C) | GI colonization and pass through | Inhibition by β-1,2-Man4 | 71 |
| SC5314 [A], CAI4, SS19-4, BAB1-4 | HeLa cells | YEPD (GYEP), synthetic dextrose | Colony counts | 110 | |
| ATCC 58716 [A] | IL-2-activated lymphocytes (m), NIH 3T3 cells (m) | SDA, RPMI 1640 (37°C) | Radioactivity (51Cr-labeled mammalian cells) | Inhibition by Man, α-MM, GlcNAc, and glucan | 81 |
a
BEC, buccal epithelial cells; VEC, vaginal epithelial cells.
b
gp, guinea pig; h, human; m, mouse; r, rat.
c
Gal, galactose; Glc, glucose; GYEP, glucose/yeast extract/peptone; SDA, Sabouraud dextrose agar; TSB, Trypticase soy broth.
d
GlcN, glucosamine; GlcNAc, N-acetylglucosamine; Man, mannose; ManN, mannosamine; α-MM, α-methlymannoside.