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. 2004 Apr;17(2):281–310. doi: 10.1128/CMR.17.2.281-310.2004

TABLE 5.

Selected reports of glycan labeling, detection and separation

Labela Detection Separationa Yr of study Reference
Perbenzoylation UV (230 nm) (RP)-HPLC 1981 63
O-benzoylation UV (230 nm) RP-HPLC 1987 62
PMP UV (245 nm) RP-HPLC 1989 113
2-AP UV (240 nm) CZE 1989 114
ANTS Fluorescence PAGE 1990 125
None UV (195 nm) CZE 1991 112
ANTS Fluorescence PAGE 1991 127
ANTS UV (214 nm) CZE 1993 53
Fluorescence
2-AP UV (200 and 230 nm) HILIC 1994 4
AMAC Fluorescence PAGE 1994 126
AMAC and ANTS Fluorescence PAGE 1994 123 (R)b
AMAC and ANTS Fluorescence PAGE 1994 124 (R)
APTS Fluorescence CZE 1995 51
2-AP UV (320 nm) CE-HPLC 1995 77
APTS Fluorescence CGE 1995 97
Various Various Various 1996 75 (R)
2-AB Fluorescence HPLC (NP and RP) 1996 96
N-(4-Aminobenzoyl)-l-glutamic acid UV (291 nm) CZE 1997 223
2-AB and 2-aminobenzoic acid Fluorescence HPAEC 1998 8
PAD NP-HPLC
AMAC Fluorescence UHV-MECC 2000 118
ANTS and APTS Fluorescence Electrophoresis 2000 228 (R)
O-(4-Nitrobenzyl)hydroxylamine Fluorescence RP-HPLC 2001 229
p-Aminobenzoic acid ethyl ester UV (340 nm) Cellulose chromatography RP-HPLC 2001 267
p-Sulfophenyl hydrazone UV (200 nm) CZE 2001 309
a

Abbreviations: 2-AB, 2-aminobenzamide; 2-AP, 2-aminopyridine; AMAC, 2-aminoacridone; ANTS, 8-aminonaphthalene-1,3,6-trisulfonic acid; APTS, 1-aminopyrene-3,6,8-trisulfonic acid; CE, capillary electrophoresis; CGE, capillary gel electrophoresis; CZE, capillary zone electrophoresis; HILIC, hydrophilic interaction liquid chromatography; HPAEC, high-pH anion-exchange chromatography; MECC, micellar electrokinetic capillary chromatography; NP, normal phase; PAD, pulsed amperometric detection; RP, reversed phase; UHV, ultrahigh voltage.

b

R indicates review article.