TABLE 5.
Selected reports of glycan labeling, detection and separation
| Labela | Detection | Separationa | Yr of study | Reference |
|---|---|---|---|---|
| Perbenzoylation | UV (230 nm) | (RP)-HPLC | 1981 | 63 |
| O-benzoylation | UV (230 nm) | RP-HPLC | 1987 | 62 |
| PMP | UV (245 nm) | RP-HPLC | 1989 | 113 |
| 2-AP | UV (240 nm) | CZE | 1989 | 114 |
| ANTS | Fluorescence | PAGE | 1990 | 125 |
| None | UV (195 nm) | CZE | 1991 | 112 |
| ANTS | Fluorescence | PAGE | 1991 | 127 |
| ANTS | UV (214 nm) | CZE | 1993 | 53 |
| Fluorescence | ||||
| 2-AP | UV (200 and 230 nm) | HILIC | 1994 | 4 |
| AMAC | Fluorescence | PAGE | 1994 | 126 |
| AMAC and ANTS | Fluorescence | PAGE | 1994 | 123 (R)b |
| AMAC and ANTS | Fluorescence | PAGE | 1994 | 124 (R) |
| APTS | Fluorescence | CZE | 1995 | 51 |
| 2-AP | UV (320 nm) | CE-HPLC | 1995 | 77 |
| APTS | Fluorescence | CGE | 1995 | 97 |
| Various | Various | Various | 1996 | 75 (R) |
| 2-AB | Fluorescence | HPLC (NP and RP) | 1996 | 96 |
| N-(4-Aminobenzoyl)-l-glutamic acid | UV (291 nm) | CZE | 1997 | 223 |
| 2-AB and 2-aminobenzoic acid | Fluorescence | HPAEC | 1998 | 8 |
| PAD | NP-HPLC | |||
| AMAC | Fluorescence | UHV-MECC | 2000 | 118 |
| ANTS and APTS | Fluorescence | Electrophoresis | 2000 | 228 (R) |
| O-(4-Nitrobenzyl)hydroxylamine | Fluorescence | RP-HPLC | 2001 | 229 |
| p-Aminobenzoic acid ethyl ester | UV (340 nm) | Cellulose chromatography RP-HPLC | 2001 | 267 |
| p-Sulfophenyl hydrazone | UV (200 nm) | CZE | 2001 | 309 |
Abbreviations: 2-AB, 2-aminobenzamide; 2-AP, 2-aminopyridine; AMAC, 2-aminoacridone; ANTS, 8-aminonaphthalene-1,3,6-trisulfonic acid; APTS, 1-aminopyrene-3,6,8-trisulfonic acid; CE, capillary electrophoresis; CGE, capillary gel electrophoresis; CZE, capillary zone electrophoresis; HILIC, hydrophilic interaction liquid chromatography; HPAEC, high-pH anion-exchange chromatography; MECC, micellar electrokinetic capillary chromatography; NP, normal phase; PAD, pulsed amperometric detection; RP, reversed phase; UHV, ultrahigh voltage.
R indicates review article.