Figure 5.

Detection of viral transcripts from CI-HHV-6. (A) RT-PCR was used to detect transcripts from U38, U73, U90 and U94 in LCLs from two CI-HHV-6A and eight CI-HHV6B carriers. Two different U90-RT-PCR products were visible in RNA samples from seven LCLs (e.g. CEPH1375.11 and HapMapNA18999). These products were of the expected sizes for unspliced and spliced transcripts. (B) Sequence analysis of the shorter U90 RT-PCR product showed that the intron (110 bp in italics) between exons 4 and 5 (44) was absent confirming that the U90 RNA had undergone correct splicing. Black arrows show the positions of the U90B2F and U90B2R primers.