Table 1.
Apparent KD values for Cdc13 proteins
| Protein | KD,app,LS (pM) | KD,rel,LS | KD,app,HS (pM) | KD,rel,HS |
|---|---|---|---|---|
| Cdc13 | 13.4 ± 0.4 | 1.0 | 410 ± 40 | 1 |
| Cdc13-DBD | 3.4 ± 0.5 | 0.25 | 210 ± 20 | 0.5 |
| Cdc13-MOB-DBD | 2.3 ± 0.2 | 0.17 | 180 ± 30 | 0.4 |
| Cdc13-F539A | 140 ± 20 | 10 | 12 000 ± 2000 | 30 |
| Cdc13-Y561A | 17 ± 1 | 1.3 | 28 000 ± 6300 | 70 |
| Cdc13-K622A | 50 ± 3 | 3.7 | 3800 ± 680 | 9 |
| Cdc13-R635A | 600 ± 80 | 45 | 140 000 ± 33 000 | 300 |
| Cdc13-L91R | 5.0 ± 0.6 | 0.37 | 220 ± 50 | 0.5 |
LS, low-salt EMSA conditions; HS, high-salt filter binding conditions.
KD,rel is relative to Cdc13 for the corresponding binding assay. Errors are standard error of the mean, n ≥ 3.