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. 2013 Sep 19;42(1):475–484. doi: 10.1093/nar/gkt843

Table 1.

Apparent KD values for Cdc13 proteins

Protein KD,app,LS (pM) KD,rel,LS KD,app,HS (pM) KD,rel,HS
Cdc13 13.4 ± 0.4 1.0 410 ± 40 1
Cdc13-DBD 3.4 ± 0.5 0.25 210 ± 20 0.5
Cdc13-MOB-DBD 2.3 ± 0.2 0.17 180 ± 30 0.4
Cdc13-F539A 140 ± 20 10 12 000 ± 2000 30
Cdc13-Y561A 17 ± 1 1.3 28 000 ± 6300 70
Cdc13-K622A 50 ± 3 3.7 3800 ± 680 9
Cdc13-R635A 600 ± 80 45 140 000 ± 33 000 300
Cdc13-L91R 5.0 ± 0.6 0.37 220 ± 50 0.5

LS, low-salt EMSA conditions; HS, high-salt filter binding conditions.

KD,rel is relative to Cdc13 for the corresponding binding assay. Errors are standard error of the mean, n ≥ 3.