Table 2.

Cooperativity constants for binding Tel11-A₆-Tel11

Protein	K2 (×1018 M−2)	K1 (×109 M−1)		ΔGcoop (kcal/mol)
Cdc13	7350	52	671	−1.3
Cdc13-L91R	48 350	171	7310	−1.1
Cdc13-DBD	478 540	960	230 400	−0.4

K₁ and K₂ are the macroscopic binding constants as described by Senear and Brenowitz (41).

Positive cooperativity is indicated by K₂ > . ΔG_coop is the free energy of cooperativity, calculated with the assumption that the two binding sites are equivalent. The numbers shown are the mean of values solved for three independent data sets.