Table 2.
Protein | K2 (×1018 M−2) | K1 (×109 M−1) | ΔGcoop (kcal/mol) | |
---|---|---|---|---|
Cdc13 | 7350 | 52 | 671 | −1.3 |
Cdc13-L91R | 48 350 | 171 | 7310 | −1.1 |
Cdc13-DBD | 478 540 | 960 | 230 400 | −0.4 |
K1 and K2 are the macroscopic binding constants as described by Senear and Brenowitz (41).
Positive cooperativity is indicated by K2 > . ΔGcoop is the free energy of cooperativity, calculated with the assumption that the two binding sites are equivalent. The numbers shown are the mean of values solved for three independent data sets.