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. 2013 Sep 30;42(1):485–498. doi: 10.1093/nar/gkt864

Figure 5.

Figure 5.

Triple G substitutions in the AT1G35720 5′-UTR largely cause a decrease in the translation efficiency. (A) Sequences of triple G substitution mutants. Three nucleotides were sequentially substituted with three Gs in the 21 nt region of the AT1G35720 5′-UTR. (B) The effect of triple G substitutions on the translational efficiency. The mutant constructs of the AT1G35720 5′-UTR were co-transformed into protoplasts together with a GUS construct and protein extracts were subjected to western blot analysis using anti-GFP and anti-GUS antibodies. (C) Quantification of the translational efficiency. To quantify the translational efficiency, the signal intensity of the immunoblots shown in (B) was quantified using the multi-gauge software equipped to the LAS3000 (FUJIFILM) and the GFP levels were normalized with the GUS levels. Error bar, standard deviation (n = 3).